Biostimulation of Native Ureolytic Bacteria and Its Potential for Calcite Precipitation in Tropical Volcanic Soils

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dc.creatorBolaños-León, Michel Dayanna
dc.creatorGuerrero-Erazo, Sebastián Camilo
dc.creatorCruz, Lucio
dc.creatorNiño, Leidy
dc.creatorGiraldo-Aristizábal, Clara Inés
dc.creatorCastro, Gloria M.
dc.creatorSandoval-Ceron, D.M.
dc.date2026-06-22
dc.date.accessioned2026-06-23T06:30:15Z
dc.descriptionMicrobially Induced Calcite Precipitation (MICP) represents a sustainable alternative for soil stabilization, with the potential to improve the mechanical properties and durability of construction materials. This study aimed to determine the influence of different concentrations of urea on the biostimulation of indigenous ureolytic bacteria in a tropical volcanic soil of southwestern Colombia under laboratory conditions, monitoring ureolytic activity through changes in electrical conductivity. The methodology involved the application of treatments using urea solutions at different concentrations and a control test without urea. The response variables were ureolytic activity, pH, and electrical conductivity over time. In addition, the mineralogical and microstructural characterization of the untreated soil was performed using FTIR spectroscopy, X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), and scanning electron microscopy (SEM). Furthermore, physical tests such as the particle size distribution and Atterberg limits were conducted according to INVIAS Colombia standards. The samples treated with urea showed higher ureolytic activity values than the control, reaching maximum values of 3.15 mM and 2.17 mM urea/min at 72 hours and 48 hours, respectively, accompanied by pH values of 8.99 and 8.88. In contrast, the control test exhibited minimal ureolytic activity and no favorable conditions for ureolysis. The results indicated that the addition of urea promotes the activation of indigenous ureolytic bacterial communities in tropical volcanic soils, improving conditions suitable for the precipitation of calcium carbonate induced by bacteria from 48 hours onward. Finally, these findings conclude the great potential of biostimulation as a sustainable strategy for improving applications and soil stabilization in infrastructure and construction materials.en-US
dc.descriptionLa precipitación de carbonato de calcio inducida por microorganismos (MICP, por sus siglas en inglés) representa una alternativa sostenible para la estabilización de suelos, con el potencial para mejorar las propiedades mecánicas y durabilidad de materiales de construcción. Este estudio tuvo como objetivo determinar la influencia de diferentes concentraciones de urea sobre la bioestimulación de bacterias ureolíticas autóctonas en un suelo volcánico tropical del suroccidente colombiano bajo condiciones de laboratorio, monitoreando la actividad ureolítica mediante los cambios de conductividad eléctrica. La metodología empleada consistió en la aplicación de tratamientos con soluciones de urea a distintas concentraciones y un ensayo control sin urea, utilizando como variables de respuesta la actividad ureolítica, el pH y la conductividad a través del tiempo. Por su parte, la caracterización mineralógica y microestructural del suelo sin tratamiento se realizó mediante espectroscopía FTIR, difracción de rayos X (DRX), espectroscopía de energía dispersiva (EDS) y microscopía electrónica de barrido (MEB). Adicionalmente, se realizaron ensayos físicos de granulometría y límites de Atterberg conforme con las normas INVIAS Colombia. Los tratamientos con urea presentaron mayores valores en la actividad ureolítica respecto al control, alcanzando valores máximos de 3.15 mM y 2.17 mM urea/min a las 72 h y 48 h, respectivamente, acompañados por valores de pH de 8.99 y 8.88. En contraste, el ensayo control mostró actividad ureolítica mínima y condiciones no favorables para la ureólisis. Los resultados demostraron que la adición de urea favorece la activación de comunidades bacterianas ureolíticas autóctonas en suelos volcánicos tropicales, promoviendo condiciones adecuadas para la precipitación microbiana de carbonato de calcio desde las primeras 48 h. Finalmente, con estos hallazgos se concluye el gran potencial de la bioestimulación como estrategia sostenible para aplicaciones de mejoramiento y estabilización de suelos en infraestructura y materiales de construcción.es-ES
dc.formatapplication/pdf
dc.identifierhttps://revistas.itm.edu.co/index.php/tecnologicas/article/view/3855
dc.identifier10.22430/22565337.3855
dc.identifier.urihttps://hdl.handle.net/20.500.12622/8181
dc.languagespa
dc.publisherInstituto Tecnológico Metropolitano (ITM)en-US
dc.relationhttps://revistas.itm.edu.co/index.php/tecnologicas/article/view/3855/4149
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dc.rightsCopyright (c) 2026 TecnoLógicasen-US
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.sourceTecnoLógicas; Vol. 29 No. 66 (2026); e3855en-US
dc.sourceTecnoLógicas; Vol. 29 Núm. 66 (2026); e3855es-ES
dc.source2256-5337
dc.source0123-7799
dc.subjectactividad ureolíticaes-ES
dc.subjectbioestimulación microbianaes-ES
dc.subjectestabilización de sueloses-ES
dc.subjectmicroorganismos nativoses-ES
dc.subjectsuelos arcillososes-ES
dc.subjectureolytic activityen-US
dc.subjectmicrobial biostimulationen-US
dc.subjectsoil stabilizationen-US
dc.subjectindigenous microorganismsen-US
dc.subjectclay soilsen-US
dc.titleBiostimulation of Native Ureolytic Bacteria and Its Potential for Calcite Precipitation in Tropical Volcanic Soilsen-US
dc.titleBioestimulación de bacterias ureolíticas autóctonas y su potencial para precipitación de calcita en suelos volcánicos tropicaleses-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeResearch Papersen-US
dc.typeArtículos de investigaciónes-ES

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