Evaluation of 3D Microstructured Scaffolds Based on PCL/Fibroin/Silver Nanoparticles as Supports for Skin Cells

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dc.creatorÁlvarez Serna, Felipe
dc.creatorMejía Suaza, Mónica Liliana
dc.creatorMoncada, María Elena
dc.creatorUribe-Yunda, Diego Fernando
dc.date2025-09-09
dc.date.accessioned2025-10-01T23:53:17Z
dc.descriptionSkin tissue engineering is a field in which living cells and scaffolds are used to treat defects. This research aimed to fabricate and evaluate three-dimensional microstructured scaffolds made of polycaprolactone (PCL), silk fibroin, and silver nanoparticles (Ag-NPs) using the wet-electrospinning technique. The methodology consisted of extracting fibroin from Bombyx mori cocoons and synthesizing Ag-NPs via chemical reduction, combining them with PCL solutions to create 3D membranes. These membranes were characterized using scanning electron microscopy (SEM), thermal analysis (TGA and DSC), FTIR spectroscopy, and water contact angle (WCA) measurements. In addition, its cytocompatibility was evaluated by MTT assay with the L929 mouse fibroblast cell line. The results showed that the inclusion of fibroin and Ag-NPs improved the hydrophilicity and cytocompatibility of the scaffolds, in accordance with the ISO 10993-5:2009 standard. The wet-electrospinning technique enabled the formation of porous structures with suitable thermal and morphological properties to mimic the extracellular matrix. Finally, it is concluded that the developed scaffolds show high potential for use as substrates for skin tissue regeneration, highlighting the need for further in vivo studies to support their application in clinical settings.  en-US
dc.descriptionLa ingeniería de tejidos de la piel es un campo en el cual se utilizan células vivas y andamios para tratar defectos. Esta investigación tuvo como objetivo fabricar y evaluar andamios tridimensionales microestructurados a partir de policaprolactona (PCL), fibroína de seda y nanopartículas de plata (Ag-NP) mediante la técnica de wet-electrospinning. La metodología empleada consistió en extraer la fibroína de capullos de Bombyx Mori y se sintetizaron Ag-NP mediante reducción química, combinándolas con soluciones de PCL para crear andamios 3D. Estos se caracterizaron a través de microscopía electrónica de barrido (SEM), análisis térmico (TGA y DSC), espectroscopía FTIR y pruebas de ángulo de contacto al agua (WCA). Adicionalmente, se evaluó su citocompatibilidad mediante el ensayo MTT utilizando la línea celular de fibroblastos de ratón L929. Los resultados mostraron que la inclusión de fibroína y Ag-NP mejoró la hidrofília y la citocompatibilidad de los andamios cumpliendo con la norma ISO 10993-5:2009. La técnica de wet-electrospinning permitió obtener estructuras porosas con propiedades térmicas y morfológicas adecuadas para imitar la matriz extracelular. Finalmente, se concluye que los andamios desarrollados muestran una alta viabilidad para su uso como sustrato para la regeneración de tejidos cutáneos, subrayando la necesidad de realizar estudios in vivo que respalden su aplicación en entornos clínicos.  es-ES
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dc.identifierhttps://revistas.itm.edu.co/index.php/tecnologicas/article/view/3345
dc.identifier10.22430/22565337.3345
dc.identifier.urihttps://hdl.handle.net/20.500.12622/7940
dc.languagespa
dc.publisherInstituto Tecnológico Metropolitano (ITM)es-ES
dc.relationhttps://revistas.itm.edu.co/index.php/tecnologicas/article/view/3345/3728
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dc.rightsDerechos de autor 2025 TecnoLógicases-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceTecnoLógicas; Vol. 28 No. 64 (2025); e3345en-US
dc.sourceTecnoLógicas; Vol. 28 Núm. 64 (2025); e3345es-ES
dc.source2256-5337
dc.source0123-7799
dc.subjectandamios tridimensionaleses-ES
dc.subjectwet-electrospinninges-ES
dc.subjectfibroína de sedaes-ES
dc.subjectnanopartículas de plataes-ES
dc.subjecttejidos dérmicoses-ES
dc.subjectthree-dimensional scaffoldsen-US
dc.subjectwet-electrospinningen-US
dc.subjectsilk fibroinen-US
dc.subjectsilver nanoparticlesen-US
dc.subjectdermal tissuesen-US
dc.titleEvaluation of 3D Microstructured Scaffolds Based on PCL/Fibroin/Silver Nanoparticles as Supports for Skin Cellsen-US
dc.titleEvaluación de andamios microestructurados 3D con base en PCL/fibroína/nanopartículas de plata como soportes para células de pieles-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeResearch Papersen-US
dc.typeArtículos de investigaciónes-ES

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