Evaluation of a Semi-Continuous Disk-Stack Centrifuge for the Harvest of Tsukamurella Paurometabola C-924 Bacterium

Pimentel Pérez, Rafael Marcos; Pérez Sánchez, Amaury; Zamora Sánchez, Jesús; López Sáez, Yovisleydis; Segura Silva, Rutdali María; Olazábal Reyes, Amparo

Evaluation of a Semi-Continuous Disk-Stack Centrifuge for the Harvest of Tsukamurella Paurometabola C-924 Bacterium

dc.creator	Pimentel Pérez, Rafael Marcos
dc.creator	Pérez Sánchez, Amaury
dc.creator	Zamora Sánchez, Jesús
dc.creator	López Sáez, Yovisleydis
dc.creator	Segura Silva, Rutdali María
dc.creator	Olazábal Reyes, Amparo
dc.date	2025-04-11
dc.date.accessioned	2025-10-01T23:53:15Z
dc.description	The disk stack centrifuge is an equipment widely used in the current biotechnological industry because of the multiple advantages it offers, the most important being its high operational flexibility, robustness, and processing speed. The objective of this research was to evaluate whether a semi-continuous disk stack centrifuge can be used to harvest Tsukamurella paurometabola strain C-924 cells, the active ingredient of the ecological bionematicide HeberNem®, to replace the tubular centrifuges currently used. The methodology used consisted of the development of a type 23 factorial statistical design, in which three input parameters were considered: feed rate [Qalim], wet weight of the diluted cell suspension to be centrifuged [PHalim], and time between discharges [tdesc]. The output parameters considered were wet weight of concentrated biomass [PHbio] and recovery percentage [ %Rec], which must have values greater than 600 g/L and 95 %, respectively, to obtain a corresponding final yield with the quality standards established for this biotechnological product. The results obtained were that the average values for PHbio and %Rec were 657.28 g/L and 97.43 %, respectively, which met the quality standards for this stage. The experimental design was optimized to determine the optimal values for the three input parameters, thus obtaining the following values: 64 L/h for Qalim, 176 g/L for PHalim, and a tdesc of 5 min. The semi-continuous disk stack centrifuge evaluated can be successfully implemented in the harvest step of the HeberNemÒ production process, thus replacing the tubular centrifuges currently employed. The statistical-mathematical programs Statgraphics Centurion® XV.II, Microsoft Excel®, and MATLAB® v7.0.1 were used for data and results processing.	en-US
dc.description	La centrífuga de discos constituye un equipo ampliamente utilizado en la industria biotecnológica actual, a causa de las múltiples ventajas que ofrece, siendo las más importantes su elevada flexibilidad operacional, robustez y velocidad de procesamiento. El objetivo de esta investigación fue el de evaluar si una centrífuga de discos del tipo semicontinua puede ser empleada para cosechar células de Tsukamurella paurometabola cepa C-924. principio activo del bionematicida ecológico HeberNem-S®, en sustitución de las centrífugas tubulares utilizadas en la actualidad. La metodología usada consistió en el desarrollo de un diseño estadístico factorial del tipo 23, en el cual se tomaron en cuenta tres parámetros de entrada: caudal de alimentación [Qalim], peso húmedo de la suspensión celular diluida a centrifugar [PHalim], y tiempo entre descargas [tdesc]. Los parámetros de salida tomados en cuenta fueron: peso húmedo de la biomasa concentrada [PHbio] y el porcentaje de recobrado [%Rec], los cuales deben presentar valores superiores a 600 g/L y 95 %, respectivamente, para obtener un rendimiento final acorde con las normas de calidad establecidas para este producto biotecnológico. Los resultados obtenidos fueron que los valores promedios para PHbio y %Rec fueron de 657.28 g/L y 97.43 %, respectivamente, los cuales cumplieron con las normas de calidad para esta etapa. El diseño experimental se optimizó para determinar los valores óptimos para los 3 parámetros de entrada, obteniéndose los siguientes valores: 64 L/h para Qalim, 176 g/L para PHalim y 5 min de tdesc. La centrífuga de discos semicontinua evaluada puede ser implementada satisfactoriamente en la etapa de cosecha del proceso de producción de HeberNem®, reemplazando por tanto las centrífugas tubulares actualmente empleadas. Se emplearon los programas estadístico-matemáticos Statgraphics Centurion® XV.II, Microsoft Excel® y MATLAB® v. 7.0.1 para el procesamiento de los datos y resultados.	es-ES
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dc.format	text/xml
dc.format	application/zip
dc.format	text/html
dc.identifier	https://revistas.itm.edu.co/index.php/tecnologicas/article/view/3263
dc.identifier	10.22430/22565337.3263
dc.identifier.uri	https://hdl.handle.net/20.500.12622/7929
dc.language	eng
dc.publisher	Instituto Tecnológico Metropolitano (ITM)	es-ES
dc.relation	https://revistas.itm.edu.co/index.php/tecnologicas/article/view/3263/3633
dc.relation	https://revistas.itm.edu.co/index.php/tecnologicas/article/view/3263/3758
dc.relation	https://revistas.itm.edu.co/index.php/tecnologicas/article/view/3263/3759
dc.relation	https://revistas.itm.edu.co/index.php/tecnologicas/article/view/3263/3760
dc.relation	/ref/M. C. Flickinger, Downstream industrial biotechnology: recovery and purification. New Jersey, U.S.A.: John Wiley & Sons, Inc., 2013. https://www.wiley.com/en-us/Downstream+Industrial+Biotechnology%3A+Recovery+and+Purification-p-9781118131244
dc.relation	/ref/R. G. Harrison, P. W. Todd, S. R. Rudge, and D. P. Petrides, Bioseparations science and engineering, 2nd ed. New York, U.S.A.: Oxford University Press, 2015. https://pdfcoffee.com/bioseparations-science-and-e-ngineering-pdf-free.html
dc.relation	/ref/Y. Chisti, “Strategies in Downstream Processing”, in Bioseparation and Bioprocessing: A Handbook, vol. 2, G. Subramanian, Ed. New York, U.S.A.: Wiley-VCH, 1998, pp. 3-30. https://doi.org/10.1016/S0734-9750(99)00012-9
dc.relation	/ref/W. W.-F. Leung, Centrifugal Separations in Biotechnology, 2nd ed. Oxford, U.K.: Elsevier, 2020. https://doi.org/10.1016/C2017-0-03265-2
dc.relation	/ref/Joseph et al., “A Scale-Down Mimic for Mapping the Process Performance of Centrifugation, Depth, and Sterile Filtration”, Biotechnology and Bioengineering, vol. 113, no. 9, pp. 1934-1941, 2016. https://doi.org/10.1002/bit.25967
dc.relation	/ref/R. Kempken, A. Preissmann, and W. Berthold, “Assessment of a Disc Stack Centrifuge for Use in Mammalian Cell Separation”, Biotechnology and Bioengineering, vol. 46, no. 2, pp. 132-138, 1995. https://doi.org/10.1002/bit.260460206
dc.relation	/ref/P. H. Chlup, D. Bernard, and G. G. Stewart, “Disc Stack Centrifuge Operating Parameters and Their Impact on Yeast Physiology”, Journal of the Institute of Brewing, vol. 114, no. 1, pp. 45-61, 2008. https://doi.org/10.1002/j.2050-0416.2008.tb00305.x
dc.relation	/ref/M. Schmidt, R. Krützfeldt, and A. Roß, “Validation of a separator CSA8 regarding sterilization for aseptic processes”, Process Biochemistry, vol. 34, no. 8, pp. 769-776, 1999. https://doi.org/10.1016/S0032-9592(98)00151-4
dc.relation	/ref/M. Iammarino, J. Nti-Gyabaah, M. Chandler, D. Roush, and K. Göklen, “Impact of Cell Density and Viability on Primary Clarification of Mammalian Cell Broth”, BioProcess International, vol. 5, pp. 38-50, 2007. https://www.bioprocessintl.com/filtration/impact-of-cell-density-and-viability-on-primary-clarification-of-mammalian-cell-broth
dc.relation	/ref/L. K. Shekhawat, J. Sarkar, R. Gupta, S. Hadpe, and A. S. Rathore, “Application of CFD in Bioprocessing: Separation of mammalian cells using disc stack centrifuge during production of biotherapeutics”, Journal of Biotechnology, vol. 267, pp. 1-11, 2018. https://doi.org/10.1016/j.jbiotec.2017.12.016
dc.relation	/ref/M. Yang, X. Liu, J. A. Howell, and H. Cheng, “Analysis and estimation/prediction of the disk stack centrifuge separation performance – Scaling from benchtop fixed rotor type to disk stack centrifuges”, Separation Science and Technology, vol. 55, no. 14, pp. 2615-2621, 2019. https://doi.org/10.1080/01496395.2019.1636820
dc.relation	/ref/L. Stoffels, A. Finlan, G. Mannall, S. Purton, and B. Parker, “Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery”, Frontiers in Bioengineering and Biotechnology, vol. 7, no. 383, pp. 1-13, 2019. https://doi.org/10.3389/fbioe.2019.00383
dc.relation	/ref/P. Esmaeilnejad-Ahranjani and M. Hajimoradi, “Optimization of industrial-scale centrifugal separation of biological products: comparing the performance of tubular and disc stack centrifuges”, Biochemical Engineering Journal, vol. 178, p. 108281, 2022. https://doi.org/10.1016/j.bej.2021.108281
dc.relation	/ref/V. Ott et al., “Qualification of a Single-Use Disk Stack Separator for Cell Separation in Mammalian Cell-Based Antibody Production”, Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1-9, 2022. https://doi.org/10.1002/cite.202200096
dc.relation	/ref/J. König, N. Janssen, and U. Janoske, “Visualization of the deposition mechanisms in disk stack centrifuges with an acrylic glass bowl top and high-speed image processing”, Separation Science and Technology, vol. 56, no. 3, pp. 640-652, 2021. https://doi.org/10.1080/01496395.2020.1728326
dc.relation	/ref/H. Salte, J. M. P. King, F. Baganz, M. Hoare, and N. J. Titchener-Hooker, “A Methodology for Centrifuge Selection for the Separation of High Solids Density Cell Broths by Visualisation of Performance Using Windows of Operation”, Biotechnology and Bioengineering, vol. 95, no. 6, pp. 1218-1227, 2006. https://doi.org/10.1002/bit.21102
dc.relation	/ref/M. Marin, J. Mena, R. Franco, E. Pimentel, and I. Sánchez, “Effects of the bacterial-fungal interaction between Tsukamurella paurometabola C-924 and Glomus fasciculatum and Glomus clarum fungi on lettuce microrrizal colonization”, Biotecnología Aplicada, vol. 27, pp. 48-51, 2010. http://scielo.sld.cu/pdf/bta/v27n1/bta05110.pdf
dc.relation	/ref/M. M. Bruzos, J. M. Campos, P. C. Chávez, R. M. Valdivia, and E. P. Vázquez, “Interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol”, Acta Agronómica, vol. 62, no. 1, pp. 52-58, 2013. http://www.scielo.org.co/pdf/acag/v62n1/v62n1a08.pdf
dc.relation	/ref/M. Marín et al., “Zea mays L. plant growth promotion by Tsukamurella paurometabola strain C-924”, Biotecnología Aplicada, vol. 30, pp. 105-110, 2013. http://scielo.sld.cu/pdf/bta/v30n2/bta04213.pdf
dc.relation	/ref/Y. L. Paneque, N. González, L. M. Crespo, J. Zamora, R. M. Segura, and A. Pérez, “Modelo matemático para predecir la estabilidad a temperaturas cercanas al ambiente de la bacteria Brevibacterium celere C-924”, Revista de Investigación, Desarrollo e Innovación, vol. 13, no. 2, pp. 367-379, 2023. https://doi.org/10.19053/20278306.v13.n2.2023.16841
dc.relation	/ref/P. F. Stanbury, A. Whitaker, and S. J. Hall, Principles of Fermentation Technology, 3rd ed. Oxford, U.K.: Butterworth-Heinemann, 2017. https://doi.org/10.1016/C2013-0-00186-7
dc.relation	/ref/
dc.rights	Derechos de autor 2025 TecnoLógicas	es-ES
dc.rights	https://creativecommons.org/licenses/by-nc-sa/4.0	es-ES
dc.source	TecnoLógicas; Vol. 28 No. 62 (2025); e3263	en-US
dc.source	TecnoLógicas; Vol. 28 Núm. 62 (2025); e3263	es-ES
dc.source	2256-5337
dc.source	0123-7799
dc.subject	bioingeniería	es-ES
dc.subject	aplicaciones industriales	es-ES
dc.subject	procesamiento de materiales	es-ES
dc.subject	evaluación del desempeño	es-ES
dc.subject	equipamiento de producción	es-ES
dc.subject	bioengineering	en-US
dc.subject	industry applications	en-US
dc.subject	materials processing	en-US
dc.subject	performance evaluation	en-US
dc.subject	production equipment	en-US
dc.title	Evaluation of a Semi-Continuous Disk-Stack Centrifuge for the Harvest of Tsukamurella Paurometabola C-924 Bacterium	en-US
dc.title	Evaluación de una centrífuga de discos semicontinua para la cosecha de la bacteria Tsukamurella Paurometabola cepa c-924	es-ES
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.type	Research Papers	en-US
dc.type	Artículos de investigación	es-ES