Extraction of Antioxidants from Blueberries (Vaccinium Corymbosum): Effect of Green Solvents on Total Phenolic Content, Antioxidant Capacity and Electrochemical Behavior

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dc.creatorBedoya-Cataño, Juan Fernando
dc.creatorRamón-Palacio, Carolina
dc.creatorGil-Garzón, Maritza Andrea
dc.creatorRamírez-Sánchez, Carolina
dc.date2022-06-01
dc.date.accessioned2025-10-01T23:52:47Z
dc.descriptionIn this study the effect of green solvents such as ethanol/water (EtOH/H2O), acetic acid/water (CH3COOH/H2O), and ethanol/acetic acid/water (EtOH/CH3COOH/H2O) was evaluated on polyphenols extraction, specifically anthocyanins that come from Vaccinium corymbosum specie, to determine its antioxidant capacity using electrochemical behavior. Total anthocyanin content (CAT) and total polyphenols content (CPT) were determined in the extracts employing spectrophotometric techniques and antioxidant capacity (CA) through electrochemical behavior using cyclic voltammetry. When EtOH/H2O is used as a solvent system, a higher polyphenols extraction is achieved, (696.46 ± 26.55) mg equivalent of gallic acid /100 g in fresh basis sample (bh), with EtOH/CH3COOH/H2O solvent mixture, more content of anthocyanins was extracted, (82.09 ± 2.59) mg equivalent of cyaniding-3-glucoside/ 100 gbh of sample, which agrees with the antioxidant capacity by cyclic voltammetry, where the highest value was obtained for the EtOH/CH3COOH/H2O mixture (270.96 ± 10.74) µmol equivalent of gallic acid /100 gbh of sample), i.e., higher concentration of monomeric anthocyanins within the total polyphenol content were oxidized at the working electrode. There are significant differences between the effect of solvent evaluation in the extraction method on each average value achieved in evaluating aspects (CPT, CAT, and CA) with a confidence level of 95 %. Additionally, there is a “strong” positive correlation between CAT and antioxidant capacity (r = 0.95).en-US
dc.descriptionEl presente estudio tuvo como objetivo evaluar el efecto de solventes verdes como el etanol/agua (EtOH/H2O), ácido acético/agua (CH3COOH/H2O) y la mezcla etanol/ácido acético/agua (EtOH/CH3COOH/H2O) en la extracción de polifenoles, específicamente antocianinas, provenientes de la especie Vaccinium corymbosum, con el fin de determinar su capacidad antioxidante mediante el comportamiento electroquímico. Para los extractos obtenidos se determinó el contenido de antocianinas totales (CAT) y contenido de polifenoles totales (CPT) por medio de técnicas espectrofotométricas y la capacidad antioxidante (CA) mediante el comportamiento electroquímico determinado a través de voltamperometría cíclica. Los resultados obtenidos indican que, si bien cuando se tiene EtOH/H2O como solvente se alcanza una mayor extracción de polifenoles, (696.46 ± 26.55) mg equivalente de ácido Gálico/100 g de muestra de base húmeda (bh), con la mezcla de solventes EtOH/CH3COOH/H2O se logra una mayor extracción de antocianinas (82.09 ± 2.59) mg equivalentes de cianidina-3-glucósido/100 g bh de muestra, lo cual concuerda con la capacidad antioxidante por voltamperometría cíclica, donde el mayor valor se obtuvo para la mezcla EtOH/CH3COOH/H2O (270.96 ± 10.74) µmol equivalente de ácido gálico/g bh de muestra), es decir que mayor concentración de antocianinas monoméricas dentro del contenido de polifenoles totales fueron oxidadas en el electrodo de trabajo. En este orden de ideas, se puede concluir que existen diferencias significativas entre el efecto que tuvo la evaluación de los solventes en el método de extracción sobre cada uno de los valores promedios alcanzados para los aspectos evaluados (CPT, CAT y CA), con una confianza del 95 %. Además, hay una “fuerte” correlación positiva entre el CAT y la capacidad antioxidante (r = 0.95).es-ES
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dc.identifierhttps://revistas.itm.edu.co/index.php/tecnologicas/article/view/2277
dc.identifier10.22430/22565337.2277
dc.identifier.urihttps://hdl.handle.net/20.500.12622/7819
dc.languagespa
dc.publisherInstituto Tecnológico Metropolitano (ITM)es-ES
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dc.rightsDerechos de autor 2022 TecnoLógicases-ES
dc.sourceTecnoLógicas; Vol. 25 No. 53 (2022); e2277en-US
dc.sourceTecnoLógicas; Vol. 25 Núm. 53 (2022); e2277es-ES
dc.source2256-5337
dc.source0123-7799
dc.subjectAntioxidant activityen-US
dc.subjectElectrochemical analysisen-US
dc.subjectAnthocyaninsen-US
dc.subjectGreen Solventsen-US
dc.subjectVaccinium corymbosumen-US
dc.subjectActividad antioxidantees-ES
dc.subjectanálisis electroquímicoes-ES
dc.subjectantocianinases-ES
dc.subjectsolventes verdeses-ES
dc.subjectVaccinium corymbosumes-ES
dc.titleExtraction of Antioxidants from Blueberries (Vaccinium Corymbosum): Effect of Green Solvents on Total Phenolic Content, Antioxidant Capacity and Electrochemical Behavioren-US
dc.titleExtracción de antioxidantes de los arándanos (Vaccinium corymbosum): efecto de solventes verdes sobre polifenoles totales, capacidad antioxidante y comportamiento electroquímicoes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeResearch Papersen-US
dc.typeArtículos de investigaciónes-ES

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